The Milford man accused of striking a Milford woman with his car and killing her in an apparent road rage incident in Hopkinton will continue to be held without bail, according to multiple news sources.

Ryan Sweatt, 36, was indicted on charges of first degree murder, multiple counts of assault and battery with a dangerous weapon and operating a motor vehicle to endanger in connection with the death of Destini Decoff, 26, on April 4. He has pleaded not guilty to all charges.

Sweatt appeared in Middlesex Superior Court on Tuesday. The magistrate judge ordered that Sweatt be held without bail but allowed him to appeal the ruling. He is due back in court Sept. 4.

Decoff’s family wore shirts bearing an image of her face to the court hearing, according to a news report.

Sweatt was driving a Honda Civic in the vicinity of Cornell’s Irish Pub around 6:25 p.m. on April 4. According to court documents, Sweatt told police he was driving home from work when the car Decoff was in pulled in front of him and slammed on the brakes. He said Decoff and four men then jumped out of their vehicle, surrounding and threatening him.

Surveillance video from the pub showed Sweatt’s car driving at a group of people who jumped out of the way. Sweatt’s car made a U-turn and hit Decoff, “propelling her into the air and down the road approximately 60-75 feet,” a prosecutor said in court.

After being on life support for two days at UMass Memorial Medical Center in Worcester and undergoing multiple surgeries, Decoff succumbed to her injuries.

Derege Demissie, Sweatt’s attorney, said that he didn’t hit Decoff intentionally. Sweatt claimed that the occupants of the car were armed with knives and that he was fearful for his life. An unnamed Hopkinton police officer at the scene said that Sweatt yelled, “They’re trying to kill me.”

“This is not a road rage case as reported by the media,” Demissie wrote in a previous statement in May. “This was an accident that resulted when Ryan tried to get away from the group of people who came out of their car to attack him. He did not see the person who was struck.”

Another news report indicated that Sweatt claimed that two of the occupants of the other car had knives and tried to confront him. The prosecutor argued that this account was inconsistent with the surveillance video obtained from the pub showed as well as what witnesses shared with the prosecutor.

At Sweatt’s last court appearance on April 30, the families of Decoff and Sweatt engaged in a fight in a hall at Framingham District Court.

Anyone with any information on the incident is asked to call Hopkinton Police Department Sgt. Gregg DeBoer at 508-497-3401.